2019-02-19 18:32:282018-02-13T15:03:58.000Z
geneLength
1353
1350
Gene
Coordinates
2,530,354 2,531,706
2,530,354 → 2,531,706
The protein
Paralogous protein(s)
[[this]]
[[protein|YotE]]
Biological materials
Expression vectors
GP1478 (chromosomal ''accC''-Strep fusion, ''aphA''3), purification from ''B. subtilis'', for [SW|SPINE], available in [SW|Jrg Stlke]'s lab
pGP1025, N-terminal Strep-tag, based on [SW|pGP172], purification from ''E. coli'', available in [SW|Jrg Stlke]'s lab
Biological materials
GFP fusion
GP1491 (spc, based on [SW|pBP43]), available in [SW|Jrg Stlke]'s lab
GP1491 (spc, based on [SW|pBP43]), available in [SW|Jörg Stülke]'s lab
Biological materials
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jrg Stlke]'s lab
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab
Biological materials
FLAG-tag construct
GP1483 (''spc'', based on [SW|pGP1331]), available in [SW|Jrg Stlke]'s lab
GP1483 (''spc'', based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab
Biological materials
Expression vector
GP1478 (chromosomal ''accC''-Strep fusion, ''aphA''3), purification from ''B. subtilis'', for [SW|SPINE], available in [SW|Jörg Stülke]'s lab
pGP1025, N-terminal Strep-tag, based on [SW|pGP172], purification from ''E. coli'', available in [SW|Jörg Stülke]'s lab